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General information
The laboratory was founded by the project “Biotechnology of New Materials” in SibFU, Institute of Fundamental Biology and Biotechnology, which had won the competition for the mega-grants of Russian Federation Government within the framework of the program to support research in Russian higher education institutions, supervised by the world leading scientists.
 
The project is focused on the establishment of the Center of biotechnology excellence; it includes fundamental research, educational and innovative activities in the field of biotechnology of new biomaterials.
 
The project is fulfilled by the Basic department of Biotechnology, involving the department for Medical Biology and others from SibFU, and resulted with the creation of the Lab of New Materials.
Энтони Дж. Сински (21Кб)
The project is supervised by the world-class leading scientist -Prof. Anthony J. Sinskey, famous in the field of biotechnology of molecular genetic, metabolomics and microbiology. Prof. Sinskey has significant experience in research and realization of his ideas in the field of metabolic engineering, aimed to the obtaining of target products, focusing on fundamental physiology, biochemistry and molecular genetics.
 

 
 
 
3rd stage
The 3rd stage of the mega-project “Biotechnology of new materials” under the supervision of the leading scientist Anthony John Sinskey has been completed.
From April 1 till June 30, 2011 in full compliance with the schedule research, educational organizational work was performed.
The following results were obtained:
The research team constructed experimental specimen of matrices in the form of microparticles out of highly purified samples of biodegradable polymers (PHA) of various composition, which were synthesized and characterized at Stage 1, for drug storage and for cellular and tissue engineering (in form of films, membranes of pressed three-dimensional forms and non-woven cloth formed by ultra-thin fibres).
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It was observed using the colouration of the cells by Romanovsky, DAPI fluorescent probe for DNA and MMT test that in the culture of fibroblasts of the NIH 3T3 line mouse PHA matrices possess high biocompatibility and do not show any cytotoxicity at the direct contact with cells.
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By adhesive properties and ability to maintain cells proliferation they are comparable with polystyrene and exceed the products made out of polylactic acid.
Experiments on the laboratory animals show that matrices in the form of microparticles from 200 nm to 50 microns, films and membranes from 0.1 to 0.6 mm thick, three-dimensional forms up to 0.5 cm in diameter obtained by various methods (correspondingly by microdropping, evaporation of the solvent, direct cold pressing) and implanted subcutaneously, intramuscularly and intraorganically do not provoke any negative reactions in the blood system, tissues and organism.
Biodegradation of PHA matrices in vivo depends on the chemical structure of the polymer, geometry of the product and the place of implantation. It was determined that the mechanism of PHA biodegradation consists in leaching of the polymer, mainly from the surface, without forming local harsh defects and significant loss of strength; it is done by humoral and cellular ways with participation of macrofags and giant cells of foreign bodies. PHA are resistant to the effects of biological environment and can be used for functioning in vivo from several months (microparticles and films) to a year and more (pressed three-dimensional matrices).
”Technical conditions for copolymer 3- hydroxybutyric and 4-hydroxybutyric acids ТУ 2200-005-03533441-2011)were developed and registered at Federal State Institution “Krasnoyarsk Centre of Standardization and Metrology”.
Educational component: 60 % of students study by credit rating system; 90% of educational programmes were re-developed for the Standard of the 3rd Generation; an educational and methodological complex for a big practical course was developed including a tutorial, programme, methodological guidelines for students’ independent work, organizational and methodological guidelines.
In cooperation with the leading scientist 1 article was published, 3 articles were submitted for publication. Totally 14 articles have been published within the framework of the project. The research team of the project became the participants of the following scientific conferences and workshops: 2nd Russian-Hellenic Symposium with Youth Scientific School “Bionanotox-2011” (Greece); International congress on marine biotechnology (China); IV International congress on industrial biotechnology (China); International conference on biopolymers and biomaterials (Italy); XLIX International scientific students’ conference “Student and science and technology progress”.
List of publications under the supervision of the leading scientist
№ п/пPublicationAuthor (s)Country, city, journal Impact factor
1Биодеградация полигидроксиалканоатов (ПГА) в восточном море и идентификация ПГА-деградирующих бактерийВолова Т. Г., Бояндин А. Н, Васильев А. Д., Карпов В. А., Кожевников И.В., Прудникова С. В., Руднев В. П., Суан Буй Ба, Зунг Ву Вьет, И. И. ГительзонРоссия, Москва, Микробиология, 2011, Т.80, №2, с.266-274.0,638
2Сравнительный анализ содержания восстановленного глутатиона и активности ферментов антиоксидантной системы эритроцитов больных аденоматозом и раком эндометрия Солопова Н. В., Титова Н.М., Пашов А.И., Савченко А.А.Россия, Владикавказ,
Владикавказ-ский медико-биологический вестник. Т.ХI, вып. 18. - С. 90-94.
0
3Прооксидантный и антиоксидантный статус крови больных почечно-клеточным ракомГерасименко М.Н., Титова Н.М., Зуков Р.А., Дыхно Ю.А., Пургина И.В., Перетока Е.С., Дерговец Д.М.Россия, Красноярск, Сибирское медицинское обозрение. - 2010. - №6. - С. 31-34.0
4Особенности окислительной модификации белков и липидов в ткани опухоли в зависимости от гистологического варианта рака легкогоР.Н.Белоногов, Н.М.Титова, Ю.А.Дыхно, А.А.СавченкоРоссия, Красноярск, Сибирское медицинское обозрение. - 2011. - №1-2. - С. 35-37.0
5Biodiesel production from sediments of a eutrophic reservoirA.Yu.Kuchkina, M.I.Gladyshev, N.N.Sushchik, E.S.Kravchuk, G.S.KalachovaBiomass and bioenergy, 2011. V. 35, P.2280-22843,326
6Efficiency of transfer of essential polyunsaturated fatty acids Versus organic carbon from producers to consumers in a eutrophic reservoirM.Gladyshev, N.Sushchik, O.Anishchenko, O.Makhutova, V.Kolmakov, G.Kalachova, A.Kolmakova, O. DubovskayaГермания, Гейдельберг, Oecologia, 2011, V.165, P.521–5310
7Влияние температуры воды на содержание незаменимых полиненасыщенных жирных кислот в пресноводном зоопланктонеМ. И. Гладышев, В. П. Семенченко, О. П. Дубовская, О. Н. Махутова, Ж. Ф. Бусева, Н. Н. Сущик, М. А. Батуриан, В. И. Разлуцкий, Е. В. Лепская, Г. С. КалачёваРоссия, Москва, Доклады академии наук 2011, Т.437, №1, С.117-1190,265
8Влияние колонии серой цапли (Ardea Cinera L) на планктон малого озера и содержание в нем незаменимых полиненасыщенных жирных кислотА.В. Крылов, М.И. Гладышев, Д.Б. Косолапов, Н.Н. Сущик, Л.Г.Коренева, О.Н. Махутова, Д.Б. Кулаков, Г.С. Калачева, О.П. ДубовскаяРоссия, Красноярск, Сибирский экологический журнал, 2011, Т.1, С.59-68.0,127
9О люминесцентной системе светящегося гриба Neonothopanus nambiБондарь В.С., Пузырь А.П., Пуртов К.В., Медведева С.Е., Родичева Э.К., Гительзон И.И.Россия, Москва, Доклады академии наук, 2011, т.438, №5, с. 705-7070,265
10Инактивация биферментной системы светящихся бактерий NADH:FMN-оксидоредуктаза-люцифераза в желатинеБезруких А.Е., Есимбекова Е.Н., Кратасюк В.А.
Россия, Красноярск
Журнал Сибирского федерального университета. Серия Биология, 2011, т.4,с 64-740
11Влияние обработкой Н2О2О-плазмой на свойства клеточных носителей из резорбируемых полиэфиров «биопластотан»Е.Д.Николаева,
Д.Б.Гончаров, Е.И.Шишацкая
Россия, Москва, Клеточная трансплантология и тканевая инженерия, 2011, Т.6, №20
12Bioluminescent enzyme assay for the indication of plant stress in enclosed life support systemsKratasyuk V., Esimbekova E., Correll M., Bucklin R.Нидерланды,
Luminescence, 2011 (принята в печать)
1,209
13Модифицированные наноалмазы взрывного синтеза в конструировании систем биохимической индикации (на примере системы определения глюкозы)Е.С.Мамаева, А.В.Барон, А.П.Пузырь, А.Е. Буров, В.С. БондарьРоссия, Москва, Доклады академии наук, 2011, т.439, №40,265
14Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kütz IPPAS H-252N. Zhila, G. Kalacheva, T.VolovaНидерланды, J.Appl Phycol (2011) V.23, P. 47-521,018
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