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General information
The laboratory was founded by the project “Biotechnology of New Materials” in SibFU, Institute of Fundamental Biology and Biotechnology, which had won the competition for the mega-grants of Russian Federation Government within the framework of the program to support research in Russian higher education institutions, supervised by the world leading scientists.
 
The project is focused on the establishment of the Center of biotechnology excellence; it includes fundamental research, educational and innovative activities in the field of biotechnology of new biomaterials.
 
The project is fulfilled by the Basic department of Biotechnology, involving the department for Medical Biology and others from SibFU, and resulted with the creation of the Lab of New Materials.
Энтони Дж. Сински (21Кб)
The project is supervised by the world-class leading scientist -Prof. Anthony J. Sinskey, famous in the field of biotechnology of molecular genetic, metabolomics and microbiology. Prof. Sinskey has significant experience in research and realization of his ideas in the field of metabolic engineering, aimed to the obtaining of target products, focusing on fundamental physiology, biochemistry and molecular genetics.
 

 
 
 
Laboratory equipment
Name of the complex, equipment, systemDate of manufactureStart-up dateCountry of productionApplication in the laboratory
1Chromatomass spectrometer Agilent 5975 Inert20072007USAAnalysis of the elements’ structure
2Microcentrifuge Eppendorf 5417R20072007USASeparation of small amounts
3Low-temperature refrigerator U570 New Brunswick Scientific20072007USAStorage of biological samples
4Automatic autoclave MLS-3781L20072007JapanSterilization
5Rotary evaporator R/210V20072007SwitzerlandEvaporation of samples
6Thermostatic shaker-incubator Exella E-2420072007USAPeriodic cultivation
7Disinfecting automatic washer G 788320072007USAWashing and disinfection of the dishware
8Laminar box of the 2nd category of biological hazardLABCONCO – 2 un.20072007USASterile work with microorganisms and cells
9CO2-incubator New Brunswick Scientific20072007USACells incubation
10System of gel penetrating chromatography Waters Alliance GPC 20082000 Series20082008USADetermination of the molecular weight of the polymers
11Universal electromechanical testing machine Instron 556520082008Great BritainTesting of the polymer samples for strength properties
12High-speed centrifuge Avanti J-26XPI20082008USASeparation of the solid and liquid parts
13Thermal packing machine NS 100020082008GermanyPacking
14laboratory hood LABCONCO (series 070976143V)20082008USAWork with chemical reagents

Using the developed modes of fermentation and the bacteria strains (Alcaligenes eutrophus В-5786, Ralstonia eutropha В-8562, Cupriavidus eutrophus ВКПМ В-10646) the family of degradable biopolymers (polyhydroxyalkanoates, PHAs) was synthesized. With implementation of gas-liquid chromatography combined with mass-spectrometry the composition of the samples was determined: poly-3-hydroxybutyrate; copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate; copolymers of 3-hydroxybutyrate and 3-hydroxyhexanoate; copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate; three-components samples containing monomers of 3-hydroxybutyrate, 3-hydroxyvalerate and 3-hydroxyhexanoate.
Experimental polymer samples were synthesized and purified to a homogenous condition. Using X-Ray, DTA and HELC methods physical and chemical properties of the polymer samples were studied. Copolymers containing monomers of 3-hydroxyvalerate and 3-hydroxyhexanoate were characterized by the minor degree of crystallinity (50-6%) in comparison with the highly crystalline homopolymer of 3-hydroxybutyrate (76-80%). Inclusions of 4-hydroxybutyrate (if compared to 3-hydroxyvalerate and 3-hydroxyhexanoate) influence significantly the ratio of crystalline and amorphous zones in the copolymers, thus significantly decreasing the crystallinity of the latter (down to 12-40%) depending on the value of the fraction of 4-hysdroxybutyrate. The molecular weight and molecular-weight distribution of the synthesized polymer samples varied between 540 000 ± 9 600 and 1 300 000 ± 28 700 Da at polydispersity from 1.60 ± 0.03 to 2.76 ± 0.03 without clear dependence on the copolymer’s composition.
© Siberian Federal University, 2010–2013
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